Scientist, Directed Evolution

Scientist, Directed EvolutionEmbarking on a rewarding journey to tackle monumental challenges in human health. Company Description:Bullseye Biosciences is at the vanguard of directed evolution, innovating on therapeutic discovery for critical targets in oncology, immunology, and metabolism. The “Archer” platform has been established from the foundational work of our academic co-founder, Dr Chory at Duke. We seek to deploy this technology against intracellular protein-protein interactions (PPIs) which are too large for small molecules and inaccessible to biologics. PPIs are extremely well validated drivers of human disease whose potential as drug targets remains a high unmet need

Your expertise in directed evolution will not just advance our research, but also contribute significantly to the development of life-changing treatments. Role Description:Come join us as a Directed Evolution Scientist in building a new generation of drugs against historically challenging targets using our directed evolution discovery engine! This role will be instrumental in building our synthetic biology capabilities and executing our screening campaigns against challenging targets. The Archer screening platform and subsequent hit testing relies on careful design of reporter “gene circuits” with multiple inducible components for precise control of the system. The Directed Evolution Scientist would help design, validate, and calibrate these systems for high-throughput evolution screening. Working with the head of research and automation scientists, this scientist would be accountable for the design and validation of novel gene circuits against challenging human disease targetsThe directed evolution scientist would work with our automation scientists and our academic founder’s lab to implement these circuits on high-throughput continuous evolution systems. They would also have the opportunity to continue supporting these targets through early drug discovery efforts. As a member of a small start-up everyone makes major contributions to the direction and success of the company!

Key Qualifications and responsibilities:Experience in PACE or similar directed evolution and 2-hybrid techniques in E Coli systemsMolecular biology laboratory techniques including bacterial culture, PCR, cloning, protein blot analysis, assay development (western blot, ELISA, luminescence), and phage propagationExpertise with plasmid/construct design and cloning techniques. Construct design and tracking using Geneious and Benchling. Sequencing by Sanger for confirmation. Understanding of plasmid components, such as inducible promoters and RBS optimizationAttention to detail and accuracy in laboratory work including familiarity with ELN programsStrong analytical and problem-solving skillsProficiency in data analysis and graphing software, including Graphpad, Spotfire, and ExcelExperience with RNA/DNA NGS and automation is a plusAbility to work independently and as a teamGood organizational and time management skillsPhD with at least 2 years of experience, or a Master's degree with at least 4 years of experience, in synthetic biology or a related field

Joining Bullseye Biosciences means more than a job—it’s a chance to own a piece of the innovation you help create, within an equitable and inclusive workplace that celebrates each milestone alongside you. What we offer:Receive competitive salary and significant equity compensation, granting you a share in the company’s success and ownership in the innovations you help bring to lifeContribution to the company at multiple levels and help drive its growth as part of the early founding teamExperience in an early start-up environment with exposure to multiple facets of the businessOpportunities for exposure to numerous startups and founders within the thriving biotech environment of the Harvard Life Labs space

If you are ready to make a tangible impact on the future of drug discovery and thrive in a fast-paced, innovative environment, we welcome you to apply and help shape the journey of Bullseye Biosciences!We are committed to providing a workplace free of discrimination and harassment based on race, color, religion, age, gender, national origin, ancestry, physical or mental or sensory disability (including the use of a trained guide dog or service animal by a person with a disability), genetic information, actual or perceived HIV or Hepatitis C infection, medical condition (cancer or a record or history of cancer and genetic characteristics), marital status, sexual orientation, pregnancy (including childbirth and related medical conditions), disability, veteran status, political ideology, social class (including caste/caste identity), taking or requesting statutorily protected leave, status as a victim of domestic violence, sexual assault or stalking, or any other basis prohibited by applicable law.To successfully perform this job, candidates must be able to satisfactorily fulfill each responsibility. The above job description represents the necessary knowledge, skills, and abilities. Reasonable accommodations may be provided to enable individuals with disabilities to perform the described functions

Interested candidates can reach us with a CV at contact@bullseye.bio